Murder Of Roger Ackroyd Essays - Alibi, Hercule Poirot,

Murder Of Roger Ackroyd The Murder Of Roger Ackroyd Dr James Sheppard lived together with his sister Caroline in King's Abbot, a small village. He was a great doctor and when somebody died he looked to see what had happened. Mrs Ferras died on the night of the 16th - 17th September. Dr Sheppard drove there. After he had analysed the body he drove home again where he talked about the death of Mrs Ferras with his sister. Caroline pretended to know everything about the death. She thought Mrs Ferras had killed herself because she had killed her husband last year. That day Dr Sheppard met Roger Ackroyd, a good friend of his, by chance. Roger invited Dr Sheppard to his house at 7.30. He also told Dr Sheppard that it was very important. That evening Roger told James that Ralph Paton, his nephew, was in London. But James had seen Ralph this afternoon. When Roger heard that, he was very angry and pretended that he didn't know that. The next morning Caroline told him that she had seen Ralph Paton with Flora Ackroyd. They had been walking together. Dr Sheppard went into the garden. Minutes later pumpkins flew past his ears and a face looked over the fence. After the new man had excused himself he introduced himself. His name was Hercule Poirot, the new neighbour. When James walked into his house again Caroline told him that she had heard that Ralph Paton had said to a girl Mr Ackroyd had to die. Then James walked to Roger's house. When Dr Sheppard entered Ackroyd's house on this day he heard the closing of a window. But he didn't pay any attention to it. In the room stood a silver table with many weapons. Dr Sheppard was looking at them when Flora entered the room. She told him that she wanted to marry Ralph, and she showed him the ring on her finger. When Roger came into the room they talked about many things. Then Roger said that Mrs Ferras had not killed her husband. Mr Ferras had been poisoned. There was a letter from Mrs Ferras and Roger wanted to read it alone, so James walked home. At 8.00 at the gates he met a stranger who wanted to know the way to Fernly Park. Back home James wanted to go to bed, but then the telephone rang and Parker, Mr Ackroyd's butler, told him that Mr Ackroyed had been murdered. So he went to Fernly Park again. When he came into the room again, he established that the letter from Mrs Ferras had been removed. Flora said that she was the last person who had seen her uncle alive. She wanted Dr Sheppard's new neighbour Hercule Poirot, a detective, to take over the case. Poirot agreed and Dr Sheppard decided to help him. They found out that Parker couldn't be the murderer because of the footpoints under the window. They didn't belong to Parker. When Poirot walked through the garden he found a ring in the pool. Hammond, the family lawyer, thought that Ralph Paton could never kill his uncle. Poirot thought the contrary. Miss Ursula Burner, a servant, didn't have an alibi. And she wasn't saying anything. Poirot sent Dr Sheppard to talk to Mrs Ackroyd the next morning. Mrs Ackroyd didn't say it directly but she accused Miss Ursula of the murder. The next day Mr Ackroyd and Mrs Ferras were buried. In the afternoon Poirot received a telephone call that the stranger from Fernly Park had been found. His name was Charles Kent. Flora came to Poirot and told him that she had stolen money from her uncle. Poirot said to her that she couldn't be the murderer and he walked to Miss Russell, a servant too, to talk to her. She confessed to Poirot to have a son, Charles Kent. So Charles Kent wasn't the murderer either because at that time he was out with his mother. When Dr Sheppard went for breakfast Caroline told him a man came to see Poirot early this morning. So he walked to Poirot but Poirot didn't tell him who it was. But he said that Ursula Bourne was Ralph Paton's wife. Poirot sent James to invite all the people from Fernly

The Spread of Religions Essays

The Spread of Religions Essays The Spread of Religions Paper The Spread of Religions Paper Short Writing Assignment # 2 Topic: The Spread of Religions 11/20/2011 Question 1: How did Buddhism, Christianity, and Islam spread across the world, and why are they practiced so far from their origins? Answer 1: Buddhism, Christianity, and Islam spread across the world by use of four common practices. Missionaries spread the faith to new areas. These missionaries adapted their message to the existing culture of the area and adopted elements of existing religious traditions. Pilgrimage was encouraged and allowed the pilgrim to express devotion through the hardships of travel and expand their world-view. Through the use of relics, people were allowed a personal connection to the story of the religion. They are practiced all over the globe, far from their origins. One reason is forced conversion such as the conversion of the Saxons by Charlemagne under threat of death. Another is diaspora such as when the Romans expelled the Jews from Judea and they scatted all over the earth. Question 2: How did these three major world religions change and adapt to diverse cultural circumstances? Answer 2: Buddhism adapted the Chinese culture in China and flourished there to an extent that China became the center of pilgrimage and dispersion even though India was the source. It adapted to Chinese culture easily because the Chinese idea of Wuwei was very similar to the idea of Nirvana as taught by Buddha. Christianity adapted to the indigenous religions in many ways. Just a few: The Church of the Holy Sepulcher was once the site of a shrine to Aphrodite. Christmas is now the celebration of Christ’s birth even though no one knows the day he was born. European Christians absorbed their old pagan tradition of the celebration of Yule into the practice of their new religion. Sufi missionaries adopted local cultural practices into the practice of Islam to attract converts. Question 3: Why did Buddhism, Christianity, and Islam emerge when and where they did? Answer 3: Each religion spread mostly in near proximity to its source: Buddhism throughout eastern Asia from India to China then on to Japan and Korea. Islam spread from Arabia through western Asia and across North Africa. Christianity spread across the Mediterranean from Judea then through Europe during the dark ages when Europeans needed a sense of unity and stability after the loss of the same after the fall of the Roman Empire. Europeans then carried it with them when they settled the New World. Question 4: How did Buddhism, Christianity, and Islam interact with, provide justification for, and conflict with various states and empires in Afro-Eurasia? Answer 4: Buddhism became the state religion of China as the nobles embraced and patronized it. Christianity became the state religion throughout Europe after the nobles embraced it then forced the conversion of their subjects as in Kiev and the mass conversion of the Saxons under the threat of death. Islam supports trade so Arabian traders spread it throughout their travels.

The Role of Women in African Society Term Paper

The Role of Women in African Society - Term Paper Example According to various kinds of literature published on development issues by The International Monitory Fund and the World Bank, the conditions of African women living in Sub-Saharan Africa is probably the bleakest on earth (Blackden 34) One cannot but recoil in shock and revulsion at the way women have been facing the twin trauma of being denied their basic human rights that every nation is supposed to guarantee to their citizens and the misfortune of being subjugated with callous disregard and disrespect in their own families. For many African women, daily life is a nightmarish reality and sheer horror, with diseased and impoverished children and community living in appalling conditions. To add insult to injury, African women customarily face the demeaning prospect of being abandoned by their husbands whose behavior could even unreservedly turn violent. Recent observations in Africa reveal that it is not uncommon for a woman to be banished from their homes and even community, resulting in untold pain, suffering, and humiliation to the woman. (Blackden 34). Nevertheless, the story does not always end in despair and tragedy. The story revolves around Ramatoulaye, a prototype of the writer herself, and the enigma and frustration she goes through as a result of abandonment by her husband for a younger girl who happens to be his daughter's friend. The consequent events in the story poignantly weaves the destiny of a woman who prefers to stay back by her errant husband's side enduring the trauma and finds solace in her religion which she faithfully follows in spite of the ordeal she is made to undergo in a society claiming to follow the very religion she also adheres to. While the author portrays Ramatoulaye's stoic handling of her unfortunate situation, she also goes a step further and provides a dash of rebellion by the character of Aissatou, Ramatoulaye's friend, who divorces her husband and migrates to the United States to pursue a more cheerful and rational environment.  

Farmgirl Broke the Contract with Pastaman Essay Example | Topics and Well Written Essays - 500 words

Farmgirl Broke the Contract with Pastaman - Essay Example The agreement between Pastaman and Farmgirl bore all the hallmarks for the existence of adequate consideration. According to Pollock (1906), one of the most basic elements to test for consideration is the premise that something must move from the offeree to the offeror. That is, the person making the offer must be expecting something in return. In this case, Pastaman offer to buy all of Farmgirl’s produce, including the excess was the item that was moving from him to Farmgirl. On the other hand, Farmgirl’s acceptance to sell all her produce to Pastaman, and no excess to a third party without his consent was the item that was moving from her to Pastaman. However, the alibi of consideration here became subjective or destroyed, given that her agreement to the contract was an aspect of detrimental reliance (Gordley, 1997) on Pastaman’s agent who claimed that their company had never sought the enforcement of the consideration clause. Farmgirl obviously acted on the be lief that there was no consideration and she had no obligation to respect the terms of not selling her excess tomatoes to a third party. As was seen in the famous case of Tweddle v Atkinson (1861)1 if no consideration is present, then the contract may not be enforceable, even if it contains a clause to the effect that it should be enforceable. In this landmark case, the absence of consideration prevented Tweddle implying a contract between himself and Atkinson (see: TweddleVAtkinson1861). However, Powell (1790) contended that another important factor that showed consideration was forbearance. That is, the consideration is said to exist when one party accepts to fail to carry out an act. Farmgirl never agreed to seek Pastaman’s consent before selling her excess tomatoes to third parties. So there wasn’t any act of forbearance here and hence any consideration.

Demographic Trend Essay Example for Free

Demographic Trend Essay The demographic trends that will have an influential impact on the needs of human services in the future will be growth and change in the populations of seniors. Person 65 and over is expected to double in size within the next 25 years in the United States population. According the (U.S. Census Bureau, 2009), Hispanic older adults is likely to go from 2.2 million in 2004 to over 15 million by 2050. It is expected to be the largest minority amongst older people by 2028. Some current trends of human service delivery that will be impacted and accentuated will be among seniors. There will be more women than men, have increased educational levels, they will probably lead active lives, live independent and be more healthy. Changes in the population will have challenges for human services during the next 50 years. Some areas will be income assistance, health care, housing, employment, the way we take part in leisure opportunities and environmental modification. The real median income for older citizens fell 2.8% for men and 3.6% for women and incomes are expected to keep declining. Human services such as mobile meal delivery and home health care make it easy for many older individuals to stay in the homes that they own or rent. Many would like to â€Å"age in place†. Older Americans are choosing to not live with their adult children; they do not want to lose their independence. Assisted living institutions help the aged to obtain and keep their independence. They keep their personal space and have social services to aid them with leisure activities, social support and cultural values. As we enter the Human Service profession we will see clients and other professionals who are different from ourselves. They were raised in other countries, have different economic backgrounds, speak various languages and be of various ages and genders. They would have entered the United States under unique circumstances. Human Services are available to more clients of diversified natures in rural areas, the military, schools and the work place for which all of these increase clients who will need these services and the professionals who can provide them. Community-based services were first introduced to clients with mental illness and who were deinstitutionalized. Today the criminal justice system, the developmentally disabled and seniors are all a part of these services. In rural areas there will always be  barriers of service delivery which include limited availability of workers, distances between clients and those who provide the services, cost, and issues of confidentiality and in what way the care will be carried out. We as a society must find ways to tackle and progress towards a solution to keep these barriers from overwhelming us to prevent the human services that are so badly needed by many. The following is from (An In troduction to Human Services, Chapter 3). Table 3.3 Summary Points Trends The effect of urbanization in poor countries will contribute to create difficulties in meeting the basic needs of people. Demographic shifts in the United States raise questions about immigration policies, language, employment and entitlement programs. One important shift is the growth and change of the older population, which indicates an increase in the number of seniors as well as changes in characteristics. Economic downturns create human service challenges for individuals and families. Clients will remain active participants in human service delivery. Advocacy as a helping skill continues to increasingly important. The Mental Health Patient’s Bill of Rights covers issues such as the right to know, confidentiality, choice, determination of treatment, nondiscrimination and treatment review. Through the use of all professionals equally sharing the burden of distributing these services as needed, will depend on the cooperation of all those involved whether it is direct contac t or through the use of referrals of other organizations. An Introduction of Human Services, Seventh Edition, Chapter 3, Human Services Today. The U.S. Census Bureau (2009) _ HYPERLINK Http://www.nationalhumanservices.org _Http://www.nationalhumanservices.org_

The Story of Electricity Essay -- Energy

In a recent newspaper article (Guernsey electricity supply ‘more’ expensive in May 2012) it stated that electricity costs are going to increase and supplies would become unreliable or erratic. It is obvious that this will cause a considerable distress and discomfort for the residents of Guernsey because electricity is needed for warmth and lighting. The purpose of this essay is to describe how and why electricity has become such an important part of people’s lives. By way of background, it is important to state what electricity actually means. According to The Need Project (Electricity: The Mysterious Force), electricity simply means electrons in motion. These electrons are tiny particles found in atoms. An atom consists of protons and neutrons. Protons and electrons are attracted to each other are attracted by each other and carry and electric charge. The proton is positively charged while the electron is negatively charged. According to The Electricity Forum: Electricity history 2012, electricity was first found in ancient Greece by Greek Philosophers over two thousand years ago. They discovered that when amber and cloth are rubbed against each other, there tends to be a form of attraction between the two. They called it the basics of electricity. Also, the article stated that many electricity-related discoveries were made during that period. In addition, Kowalski, K. M. 2009 stated that before the early 1900’s the source of light was from candles and oil and gas lamps. However, it was very dangerous as it could cause fire explosions. There have been three respected people whom in the past contributed greatly to the development of electricity. Firstly, Benjamin Franklyn. He was an American scientist who established... ...ion=2&contentSet=GALE%7CA211235661&&docId=GALE|A211235661&docType=GALE&role=ITOF&docLevel=FULLTEXT on 24th May, 2012. Sharman, F and Parker, B. The History of Electric Supply in the Area. Retrieved from http://www.localhistory.scit.wlv.ac.uk/articles/electricity/history1.htm on 22nd June, 2012. The Electricity Forum: Electricity History. Retrieved from http://www.electricityforum.com/electricity-history.html on 20th May, 2012. The Electricity Forum: Dangers of Electricity. Retrieved from http://www.electricityforum.com/dangers-of-electricity.html on 24th May, 2012. The Need Project (Electricity: The Mysterious Force) retrieved from http://www.need.org/needpdf/infobook_activities/IntInfo/Elec1I.pdf on 20th May, 2012. Woodford, C. 2011 Electrical transformers. Retrieved from http://www.explainthatstuff.com/transformers.html on 22nd May, 2012.

How Does Temperature Affect Lipase

How does temperature affect the rate of reaction for Lipase? As the temperature increases, so will the rate of enzyme reaction. However, as the temperature exceeds the optimum the rate of reaction will decrease. I predict that at temperatures above 70 °C the enzyme lipase will become denatured and at temperatures below 10 °C the enzyme will become inactive. Since lipase operates within the human body I’d also predict that its optimum temperature would be around human body temperature which is approximately 37 °C.I predict that before the optimum temperature the rates will gradually increase and preceding the optimum there will be a drastic decrease in rate until the enzyme is denatured. I predict that the rate of enzyme activity at 45 °C will be half that of 30 °C. I predict that the rate of enzyme activity at 45 °C will be half that of 30 °C. Diagram courtesy of: http://www. rsc. org/Education/Teachers/Resources/cfb/enzymes. htm Diagram courtesy of: http://www. rsc. org/Education/Teachers/Resources/cfb/enzymes. htmIn my controlled assessment I will be investigating the activity of lipase on milk fat at various temperatures so that I can then find an accurate temperature as to when the enzyme works at its optimum; when it becomes inactive and when it denatures. To find when the enzyme denatures is to find out when the bonds of this protein disintegrate and henceforth disable the enzyme from being of any further use. When these bonds break, the protein starts to unfold and loses some its properties. For example, a denatured protein usually becomes less soluble. As an enzyme, it will lose its ability to function as a catalyst.If the stress that is causing the denaturation continues, other changes may occur. Now that the normal structure of the protein is gone, new bonds may be formed, giving it a different shape. The bonds broken in a denatured enzyme is that of which links the polymers to form the amino acids. This means that if lipase were to denature at the higher temperatures it will then cause inactivity in breaking down the fat of the milk hence leaving the unchanged. In this investigation, however, there are numerous factors as to what can affect the investigations results.First of all, the temperature of the room can play a role in altering the results as it can change the temperature of both the solution and lipase. Moreover if one were to move the solution or lipase to another part of the room, or to carry out the investigation on a different day, the temperature surrounding the solution and lipase will change and henceforth change the temperature of the solution and lipase. Secondly, if the temperature of the water bath isn’t precisely the temperature it is supposed to be then, as expected, would change.Thirdly, the age of the contents can affect the concentration of the substrates which would then decrease the rate of reaction with lipase. Finally, there is the factor of human error, as we may not be capable of making perfect measurements consistently the amounts of each component will inevitably change, which would in effect change the results. Of this investigation our independent variable will be the rate of reaction, which we will measure by timing how long it would take for the solution to turn white after having the lipase poured in.Our dependent variable will be the time it takes for the solution to turn pink after having the lipase poured in. Our controlled variable is that of will be all other factors. Enzyme Diagram courtesy of http://students. cis. uab. edu/clight/finalprojectwhatisanenzyme. html Diagram courtesy of http://students. cis. uab. edu/clight/finalprojectwhatisanenzyme. html An enzyme is a molecule that changes the speed of reactions. Enzymes can build up or break down other molecules. The molecules they react with are called substrates; enzymes are catalysts.An enzyme works by allowing a substrate, or multiple substrates, to enter the active site, which is where the reaction takes place, and then to exit in either more or less pieces then it was when it first entered. The active site is unique to a specific substrate which means that other substrates cannot react with that enzyme unless the enzyme is modified. [An active site can be altered by a non-competitive enzyme which encircles the enzyme and alters the shape of the active site which could be very dangerous. ] Diagram courtesy of: http://www. wiley. com/college/boyer/0470003790/reviews/kinetics/kinetics_effec ors. htm Diagram courtesy of: http://www. wiley. com/college/boyer/0470003790/reviews/kinetics/kinetics_effectors. htm Note that the enzyme remains unchanged so that more of the some substrates can react. Note that the enzyme remains unchanged so that more of the some substrates can react. Structure Proteins are polymers made by joining up small molecules called amino acids. Amino acids and proteins are made mainly of the elements carbon, hydrogen, oxygen and nitrogen. Pro tein Protein Amino Acid Amino Acid Each gene acts as a code, or set of instructions, for making a particular protein.They tell the cell what to do, give its characteristics, and determine the way its body works. Each protein has a unique sequence of amino acids. This means that the number and order of amino acids is different for each type of protein. The proteins fold into different shapes. The different shapes and sequences give the proteins different functions, e. g. keratin are a fibrous protein found in hair and nails. If the gene has even the slightest of disorder within its sequence it could lead to an inaccurate order of amino acids and so a faulty protein or in our case faulty enzymes.Substrate concentration An enzyme has an active site where it binds the molecule (or molecules) it acts upon; the enzyme then catalyses a chemical reaction involving that molecule (or those molecules). That molecule (or those molecules) is called the enzyme's substrate. So the substrate concen tration is the concentration of the molecules an enzyme works on. Diagram courtesy of http://biochemistryquestions. wordpress. co m/2008/07/15/induced-fit-model-of-enzyme-substrate-interaction/ Diagram courtesy of http://biochemistryquestions. wordpress. o m/2008/07/15/induced-fit-model-of-enzyme-substrate-interaction/ In general, if there is an increase in substrate concentration, then more enzymes will be catalysing the chemical reaction and the overall rate of reaction will increase. It will continue to increase until all enzymes are actively binding substrate (called saturation), at which point no further increase in rate can occur, no matter how high you raise the substrate concentration. In my investigation into enzyme response to temperature this graph will be of relevant. Diagram courtesy of: http://www. sc. org/Education/Teachers/Resources/cfb/enzymes. htm Diagram courtesy of: http://www. rsc. org/Education/Teachers/Resources/cfb/enzymes. htm Denatured Denatured Denaturing Denaturing Less kinetic energy so the reaction slows down. Less kinetic energy so the reaction slows down. This graph illustrates the response that rate of enzyme activity has at various temperatures. At lower temperatures the rate is very low as there isn’t enough kinetic energy for the enzyme to work at its optimum, then you of course have the enzymes temperature optimum where the enzyme works best at.Finally you have the denaturing of the enzyme which eventually halts with the enzyme being completely denatured where it then will never have any activity. Collision Theory For a chemical reaction to occur, the reactant particles must collide. But collisions that do not have enough energy do not produce a reaction. The particles must have enough energy for the collision to be successful in producing a reaction. The rate of reaction depends on the rate of successful collisions between reactant particles. So the less successful collisions that occurs the less products created. D iagram courtesy of: ttp://www. worthington-biochem. com/introbiochem/tempeffects. html Diagram courtesy of: http://www. worthington-biochem. com/introbiochem/tempeffects. html The reason as to why particles may have or may not have enough energy to create products depends on the amount of kinetic energy in the particles. Hence why at lower temperatures the enzyme becomes inactive as there isn’t a high enough temperature to create the necessary kinetic energy to create the products. As the temperature increases so does the rate which is due to more kinetic energy and hence more successful collisions. H An enzyme can also denature upon extreme pHs. with the extreme pH’s being 1 and 14, the enzyme would denature due to the hydrogen acids within the pH’s damaging the amino acid bonds within the enzyme. By damaging these bonds, the amino acids break apart, this in turn means that the enzyme’s active site will lose its shape, resulting in the denaturing of the enzyme. Henceforth, the optimum pH is in the middle of the pH spectrum as neutral pHs are unable to damage the bonds of the amino acids keeping the enzyme capable of reaction.Preliminary Method a. Get a test tube for each temperature being investigated. b. Add 5 drops, using a pipette, of phenolphthalein to the test tube. c. Measure out 5 cm3  of milk using a measuring cylinder and add this to the test tube. d. Measure out 7 cm3  of sodium carbonate solution using another measuring cylinder and add this to the test tube. The solution should be pink. e. Place a thermometer in the test tube. f. Place the test tube in a water bath and leave until the contents reach the same temperature as the water bath. g.Remove the thermometer from the test tube and replace it with a glass rod. h. Use the 2 cm3  pipette to measure out 1 cm3  of lipase from the beaker in the water bath for the temperature you are investigating. i. Add the lipase to the test tube and start the stopwatch. k. Sti r the contents of the test tube until the solution loses its pink colour. l. Stop the clock/ watch and note the time in a suitable table of results. *A control was also investigated by having a test tube with the sodium carbonate, phenolphthalein and milk but without the lipase.This is to test as to whether the solution would turn from pink to white regardless of whether the enzyme was present or not. This was the original method which was used to carry out the preliminary investigation, however upon consideration it was decided that for the real practical a slightly alternate method should be used. In our edited method we made the changes of firstly, on putting the lipase into the water bath, this was because heating up the solution instead is to investigate the effects of the temperature of the solution as oppose to how the temperature of the enzyme effects.Secondly it was decided upon that we would not stir the contents for two reasons: firstly because by stirring the solution it spread the lipase around more which in effect speed the reaction up so much that it was impossible to time; secondly, by stirring the contents it often made the solution over flow which both made a untidiness and caused the volume of the contents to decrease. Finally it was decided that we were to limit the amount of temperatures being investigated as temperatures below 22? the enzyme was inactive hence taking too long to record the time it took for the solution to turn white, at temperatures over 55? c the enzyme, the lipase enzyme would be denaturing hence taking too long to be able to record as well. Final Method a. Get a test tube for each temperature being investigated. b. Add 5 drops, using a pipette, of phenolphthalein to the test tube. c. Measure out 5 cm3  of milk using a measuring cylinder and add this to the test tube. d. Measure out 7 cm3  of sodium carbonate solution using another measuring cylinder and add this to the test tube.The solution should be pink. e. Plac e a thermometer in the test tube. f. Place the test tube, containing only the lipase enzyme, in a water bath and leave until the contents reach the same temperature as the water bath. g. Remove the thermometer from the test tube. h. Use the 2 cm3  pipette to measure out 1 cm3  of lipase from the beaker in the water bath for the temperature you are investigating. i. Add the lipase to the test tube and start the stopwatch. k. Stop the clock/ watch and note the time in a suitable table of results. A control was also investigated by having a test tube with the sodium carbonate, phenolphthalein and milk but without the lipase. This is to test as to whether the solution would turn from pink to white regardless of whether the enzyme was present or not. Such changes were made in an attempt to improve the validity of the investigation. As is in the nature of an investigation it is impossible to make the results completely accurate and precise. What we can do however is improve the reprod ucibility and reliability of our results by repeating the test multiple times.Risk Assessment Substance| Hazard| Risk| Risk rating*| Emergency action| Phenolphthalein | LOW HAZARD| Although it is not hazardous one should take precaution avoiding skin contamination. | 1| If in contact with eyes then flood eyes with water to wash it out. | Lipase| HAZARD| If in contact with skin it can cause an itch. If someone were to have an allergic reaction to lipase it could cause symptoms such as rashes. | 1| Seek emergency assistance if you believe you are having an allergic reaction to lipase. However wash it off as quickly as possible. Sodium Carbonate| IRRITANT| Sodium carbonate contributes to three major hazards: skin irritation, eye damage and internal effects. | 3| If swallowed, drink two or more glasses of water or milk. If in contact with skin use a cloth to wipe the sodium carbonate or rinse with water and if contact with eyes rinse thoroughly. | Milk| LOW HAZARD| If in contact with sk in it can cause an itch, however some people may have an allergic reaction to the substance. | 2| Acting in accordance to the severity of the reaction, one should wash it off as quickly as possible. Water| HAZARD| As the temperature of water we are to use will range between 10 °c-80 °c hot water may come in contact with us and burn ones skin. | 2| If hot water comes in contact with one’s skin one must rinse thoroughly with cold water to prevent further burning. | Test Tubes| HAZARD| If one were to drop a test tube, it would be very likely for it to smash, disintegrating over the floor which could then cut someone’s foot. | 2| If there is to be a broken test tube on the floor one must alert a member of staff and sweep the area whilst restricting anyone from crossing until one has finished clearing the area. Kettle| LOW HAZARD| If one were to knock a kettle over whilst boiling water the contents would spill and henceforth burn someone or something. | 1| Keep the kett le away from electrics and other peoples working areas. | *Risk rating out of /5 Generic precautions As in all practical’s one must always take precaution of what is at hand, moreover it is obligatory to wear goggles to protect the eyes and to reduce the risk of skin contact one can wear disposable gloves.Another precaution to take is to ensure that no obstacles obstruct your movement as one may then spill a substance or break a piece of apparatus, a basic step is to push in all stools and to stand up when you do a practical. In addition a class should always leave their bags at the back of the classroom and put aside planners and books making a clear workstation. Any spills, accidents or injuries should be dealt with immediately and inform a member of staff. Review of Evidence The shape of the graph resembles that of the rate of enzyme activity graph on page 3, an arc.With the shape of the graph being similar to an arc, it displays clearly that there is a definite optimum to the rate of lipase’s activity and the stages of inactivity and denaturing. The optimum temperature of lipase on this rate graph was the same in both my preliminary data and my real results data which was 30 °c and in both instances the shapes of the graphs do resemble that of an arc. In the preliminary graph, the range bars were rather extensive for example, at 35 °c the difference between the highest (non-anomaly) result and the lowest was 0. 13 in rate.These inaccurate results could have been due to multiple factors with the more obvious being either human error or faulty equipment. By having such a difference in results it only justified the changes which we had made for the real investigation. When looking back upon my original hypothesis, it stated that before the optimum temperature the rates would gradually increase due to the lack of kinetic energy provided from the heat. Upon reviewing the graph it is clearly illustrated that there is an increase in rate from te mperatures 22 °c-30 °c with an increase of 0. 26 in rate. I also predicted that the optimum temperature would be 37 °c, due to the fact that lipase operates in the human body and the human body’s temperature should be 37 °c. By analysing the evidence of which the graph presents it tells me that the highest rate of reaction was that of 30 °c, meaning this was the optimum temperature. Finally, I predicted that once the optimum was exceeded, the rates would begin to decrease as they cannot function at such temperatures due to the breaking in the peptide bonds that holds the amino acids together.Once this bond is broken, the enzyme is reduced to its primary structure which is just peptide bonds occurring – the functional structure of the enzyme is lost and it is no longer functional; denatured. After the optimum temperature, which was 30 °c, the rate of reaction began to decline as the temperatures increased. Henceforth, my prediction was right in saying that o nce the optimum temperature had been passed; the denaturing process would begin to take place, meaning the rates of reactions would become slower.Upon looking back at my quantitative prediction, which stated that â€Å"at 45 °c will be half that of 30 °c. † However, the decrease in rate was far more drastic then I had predicted. (Rate of 30 °c was 0. 032; rate of 45 °c was 0. 005. ) This means that the process of denaturing was far quicker than I had previously predicted which in turn means that my quantitative was incorrect. However, if I were to replace the 45 °c figure in my initial quantitative prediction with 35 °c it could then be plausible as the rate of 35 °c was 0. 011 (30 °c-0. 032. )In addition, I would further modify my initial prediction bySecondary data By analysing the provided secondary data I shall be able to further prove or disprove the evidence that I had recorded. By being able to prove my data with secondary data which has the same outcom e and conclusion it proves that that the data is repeatable as there are externally recorded results that support the results that I had recorded. Figure 4 courtesy of: http://www. currentscience. info/upload/IssuesFile/29_issues_Article%2010. pdf Figure 4 courtesy of: http://www. currentscience. info/upload/IssuesFile/29_issues_Article%2010. pdfFigure 3 courtesy of: http://www. google. co. uk/url? sa=t;rct=j;q=;esrc=s;source=web;cd=7;ved=0CGIQFjAG;url=http%3A%2F%2Fwww. diagnosisp. com%2Fdp%2Fjournals%2Fview_pdf. php%3Fjournal_id%3D1%26archive%3D0%26issue_id%3D31%26article_id%3D1135;ei=nrjEUJ2XC8HJ0AXPy4DACQ;usg=AFQjCNEb15WjPAyJMMgCDAjs3ZaorsN3qg;sig2=mf7h7XRNBjWBD3cdMS2v-w Figure 3 courtesy of: http://www. google. co. uk/url? sa=t;rct=j;q=;esrc=s;source=web;cd=7;ved=0CGIQFjAG;url=http%3A%2F%2Fwww. diagnosisp. com%2Fdp%2Fjournals%2Fview_pdf. hp%3Fjournal_id%3D1%26archive%3D0%26issue_id%3D31%26article_id%3D1135;ei=nrjEUJ2XC8HJ0AXPy4DACQ;usg=AFQjCNEb15WjPAyJMMgCDAjs3ZaorsN3qg;sig2=mf7 h7XRNBjWBD3cdMS2v-w Figure 1 (left) ; 2 (above) courtesy of: http://www. slideshare. net/wkkok1957/effect-of-temperature-on-lipase-activity-using-ph-sensor Figure 1 (left) ; 2 (above) courtesy of: http://www. slideshare. net/wkkok1957/effect-of-temperature-on-lipase-activity-using-ph-sensor Comparing the data sets As is clearly shown in all of the above figures there is a clear optimum. In terms of the optimum temperature, it ranges from 35 °c (figure 2 ; 3) to 25 °c (figure 4. Whereas in the recorded data that I had collated, it was 30 °c with the rate for 35 °c being significantly less than half of the rate for 30 °c. When comparing the rates at 20-25 °c another difference in rate had occurred as you can see in figures 1, 2, 3 ; 4 there isn’t such a sharp increase in rates whereas in my own results there is a steep increase in rate between 22 °c and 30 °c, a difference of 0. 026. ) Moreover, in terms of the temperature at which the lipase denatures also vari ed as the denatured point in figure 3 is at 50 °c whereas the temperature at which the lipase denatured in my investigation was at 55 °c.Finally in terms of the shape of the graphs you can see that in figure 2 the shape of the graph is of a rather steady contour oppose to the sharp point that is of my graph shape. The foremost reason as to what caused such differences was the fact that the secondary investigations used an alternate for example in figures 1 ; 2 the method utilised was slightly different as they used more accurate pieces of apparatus for example they used a micropipette to measure the sodium carbonate into the test tube which would ensure for far more accurate measurements then I had made.Secondly they used a pH probe a Logger Pro to detect the change in the milk which would also prove for much more accurate readings in comparison to detecting the change with the eye as we cannot see the entire of the solution and we, henceforth, could record a shorter or longer t ime to the actual figure as we would essentially be guessing as oppose to knowing when the reaction was definitely complete. On the contrary however, they only repeated each temperature 3 times so as to collect triplicate data.In conclusion I would say that by analysing secondary data it does support my data in its general trend but in terms of individual figures, inactivity and denatured points I am unable to defend and justify that my investigation is completely reproducible. I must say that in all, I would say that the reason as to why there is a difference in the primary data and secondary data is due to multiple factors such as alternate methods, alternate apparatus and an alternate working environment.However, in total, I do feel confident in saying that my results are reproducible to such an extent that it can resemble that of the actual figures and graph shape. Evaluation of errors I believe that the changes made to the preliminary method for the real investigation did impro ve the overall accuracy of the data in the real results data. However, in the results there were many outliers that were recorded, six in total. These errors and possible inaccuracies were made possible by such factors as human error, equipment error and technique rror. In terms of human error we may have made the mistake of timing the reaction wrong because the people who are timing the investigation may time it wrong. Secondly, there may be a difference in opinion in when the reaction would have fully completed as one may say that the solution still contains traces of pink yet another may say that the solution has no traces left. Finally, there could have been the human error of inaccurately measuring the portions of the solution.In terms of equipment error, sometimes the water baths were unable to heat the solution to the specified temperature of which were trying to investigate which would then have the effect of us collating alternate data to what we should have got, this would then alter our rate bars as they be higher or lower. Furthermore, there may no longer have been a real difference in the data’s even if there was supposed to be. Secondly, our portions of the solution may have been measured inaccurately as the measuring cylinders used may have not been accurate enough for us to get precise measurements.On top of this, whilst using the pipette to measure the contents into the measuring cylinder, air bubbles were created which then alter our results as we would then be measuring a different quantity as opposed to the proposed temperature. Finally such technique errors occurred such as the lipase may have not spread equally amongst the solution which would have left a section of the solution untouched by the enzyme. Furthermore as we took the lipase out of the water bath the temperature of the lipase would either increase or decrease if above or below the room temperature.To improve the accuracies and reliabilities of the data collected and to reduce the errors as is mentioned above I would make such alterations to the existing method: -To ensure that the lipase truly got to the temperature that it was supposed to be at an improvement would be as to set the temperature of each water bath 3 °c higher than what was prepared for which would make it easier for the lipase to heat up to the specified temperature. To increase the accuracy and eliminate the of measuring incorrectly the solution ingredients an improvement could be to use a syringe as oppose to a pipette as the pipette can’t measure as accurately as a syringe because whilst using the pipette bubbles where constantly created which made it incredibly difficult to then accurately measure the contents that were to be measured in. -As is the nature of foods and drinks the milk would eventually surpass the date hat it was meant to be consumed by. However this means that the bacteria within the milk may function in a different manner because the bacteria uses the lactose sugars to reproduce, they change it from â€Å"lactose sugar† into â€Å"lactose acid,† which tastes sour and it becomes a huge food borne illness risk to consume it and it must be discarded. Instead then we can use such alternatives as UHT or powdered milk as they have longer life spans because more of the bacteria is removed. To remove the factor of misjudgement whilst trying to detect as to whether the solution has lost all traces of pink an improvement can be to use a pH probe next time as the pH probe could then accurately detect once the reaction has completely finished by seeing when the figures stop changing on the pH probe. Improved Method a. Get a test tube for each temperature being investigated. b. Add 5 drops, using a pipette, of phenolphthalein to the test tube. c. Measure out 5 cm3  of milk using a measuring cylinder and add this to the test tube. . Measure out 7 cm3  of sodium carbonate solution using another measuring cylinder and add this to the test tube. The solution should be pink. e. Place a thermometer in the test tube. f. Place the test tube in a water bath and leave until the contents reach the same temperature as the water bath. g. Remove the thermometer from the test tube and replace it with a glass rod. h. Use the 3 cm3  syringe to measure out 1 cm3  of lipase from the beaker in the water bath for the temperature you are investigating. . Add the lipase to the test tube and start the stopwatch. k. Using the pH metre wait until it displays that no pink resides in the solution. l. Stop the clock/ watch and note the time in a suitable table of results. *A control was also investigated by having a test tube with the sodium carbonate, phenolphthalein and milk but without the lipase. This is to test as to whether the solution would turn from pink to white regardless of whether the enzyme was present or not. Evaluation of proceduresWhen analysing and evaluating the procedures I shall divide the section into fo ur sectors: precision, accuracy, repeatability and reproducibility. Precision refers to how well experimental data and values agree with each other in multiple tests. [1] The only evidence to demonstrate the precision of the data is the range bars. All range bars excluding 30 °c ;0. 001, however for 30 °c the range is ;0. 001. This proves that the precision of the data was quite good with the exception of the data for 30 °c.By having a small range in data it exemplifies precision of the data as they are all within a similar region of figures. However with 30 °c the data was rather spread meaning that the results for 30 °c degrees were not precise due to the fact that my range bar is rather spread when compared to the likes of the data from 22 °c where the range bar is a quarter of the size of the range bar for 30 °c. This provides me with the necessary evidence to believe that the rest of my results were precise, with the results for 30 °c being the exception.The abi lity to obtain consistent results when measuring the same part with the same measuring instrument. [2] Upon considering the repeatability of this investigation one can say that the results are most certainly repeatable as the data resembles that of which others have collated and that of the preliminary data. If one were to repeat the investigation with the improved method then the investigation is, with no doubt, repeatable as the evidence lies within the secondary data that supports the data of which I have collated. Accuracy refers to the correctness of a single measurement.Accuracy is determined by comparing the measurement against the true or accepted value. [3] Although there is nothing we can do to improve the accuracy per say, we can, for example, remove outliers that do not share any resemblance to that of the true value, we are able to make more accurate calculations as to what the average is because we are taking out a value that does not mean anything to the true value. B y doing so in my calculations it not only improved the accuracy of the results but it also exemplified how some factors could change the results so drastically.This demonstrates that although we can control most factors that alters the results we can’t completely control them as there are endless factors as to what can affect the results recorded, for example the room temperature could affect the results is could have heated or cooled the solution. By controlling the variables of which were possible to control we did all that was possible for us to do in order of making the investigation valid. Furthermore, by repeating the outliers again to get a new set of results it would provide for a more accurate average.This is something that was not done due to the lack of time Reproducibility is one of the main principles of the scientific method, and refers to the ability of a test or experiment to be accurately reproduced, or replicated, by someone else working independently. [4] I f the results were to be reproducible then it would be possible to look at secondary data and see that it closely resembles that of the results I have provided. When comparing my results to that of peers of who are carrying at the same investigation there is most certainly a resemblance in the overall shape of the graph.Although the rates may differ the general trend of the graph does suggest the same conclusion that there is a definite optimum at around 30 °c-35 °c. http://www. slideshare. net/wkkok1957/effect-of-temperature-on-lipase-activity-using-ph-sensor -this is a link to someone else’s investigation and results (Tony Hong), from this link you are able to see Tony’s investigation and results that follow a similar method as to mine. With this it is possible to see the results and henceforth make a conclusion as to whether my results are reproducible.By looking at his data, it displays clearly that the optimum temperature that he got was 35 °c whereas in my investigation it was 30 °c. Furthermore it seems as if that his rates seem to be considerably higher than that of mine. For example, at 35 °c the rate was 0. 011 whereas Tony got 0. 00038 (s-1. ) In conclusion, it could be said that although my graph does follow the general trend of having a definite optimum and the stages of inactivity and denaturing. However, the actual figures did vary from what I had collated meaning that my investigation’s results are most probably not reproducible.Outliers As is seen in the result tables the outliers have been circled which were then excluded when calculating the averages for it could completely change the course of the results for if they were to be used as valid results whilst calculating the average it would transform what the real results were originally presenting. Such outliers occurred due to infinite factors, however there were factors of which were attempted at being controlled as is mentioned in Page 1. Overall there was a total of six recorded anomalies, this not only had the effect of creating inaccuracies but also difficulties n detecting which figure was of the figures and which were of the anomalies. Although there was the option of using a 10% lean way which would provide for a fixed bracket as to which the figures can fall into, to what would the 10% lean way be from if we didn’t know which figure was the anomaly. The only way to resolve this problem would be to repeat the anomalies in order to attain figures which support the other figures better. Conclusion In summary I believe that the investigation that I had carried out was rather successful in that it proved that there is a definite optimum temperature as to when lipase works at its best.It also illustrated the stages of inactivity and denaturing. However, the theoretical optimum should be approximately 37 °c; the optimum that was recorded was 30 °c which would suggests that there were systematic errors. If I were to repeat th e same investigation again I would most certainly make some alterations in the method so as to improve the overall validity of the investigation. Such alterations to the method would be to use more accurate apparatus such as a pH probe to detect the reaction and a micropipette so as to improve the accuracy of the measurements of the solution contents. Bibliography http://www. lideshare. net/wkkok1957/effect-of-temperature-on-lipase-activity-using-ph-sensor -How will changing the temperature affect the rate of lipase activity of digesting milk fat into fatty acid and glycerol measured using a pH probe? 03/12/2012 Rating: A university degree investigation that seems rather professional. The investigator is an IB student. 8/10 http://www. worthington-biochem. com/introbiochem/tempeffects. html-Introduction to Enzymes. /11/2012 Rating: Worthington Biochemical Corporation was founded in 1947 for the purpose of preparing enzymes for the growing biochemical research community.The article was excerpted from a very popular Worthington publication which was originally published in 1972 as the Manual of Clinical Enzyme Measurements. While some of the presentation may seem somewhat dated, the basic concepts are still helpful for researchers who must use enzymes but who have little background in enzymology. 9/10 http://www. rsc. org/Education/Teachers/Resources/cfb/enzymes. htm –Enzyme. /11/2012 Rating: The site is aimed at students above the age of 16 who are taking Biology for further studying. It is also of use to first year undergraduates studying biology.It assumes that you have studied some chemistry. The website is supported by the Royal Society of Chemistry. 10/10 http://biochemistryquestions. wordpress. co m/2008/07/15/induced-fit-model-of-enzyme-substrate-interaction/ -Induced fit model of Enzyme-Substrate interaction. /11/2012 Rating: The biochemistry questions site is a free Biochemistry Question Bank for medical students and FMG. It is a forum where one asks a question for someone else to answer your question. It is an open source meaning that answers can derive from anywhere. 6/10 http://www. iley. com/college/boyer/0470003790/reviews/kinetics/kinetics_effectors. htm -Elementary Kinetics. /11/2012 Rating: This site is intended to supplement and extend the critical concepts presented in the Boyer textbooks. Both students and instructors at the site are encouraged to explore the world of biochemistry through multi-media. http://students. cis. uab. edu/clight/finalprojectwhatisanenzyme. html -What are Enzymes? /11/2012 Rating: Virtual chembook Elmhurst College. The site is based upon Charles E. Ophardt, Elmhurst College, findings.There is very little background to the website besides the fact that it was founded in 2003 by Charles E. Ophardt himself. 7/10 http://www. rsc. org/Education/Teachers/Resources/cfb/enzymes. htm -Enzymes. /11/2012 Rating: To see the rating for this website please look back through the bibliography to find the rating for the same website. [1] http://chemistry. about. com/od/chemistryglossary/g/Precision-Definition. htm -Precision Definition. 03/12/2012 Rating: The definition was written by Anne Marie Helmenstine, Ph. D. 10/10